DPDPE vs TFF3

Head-to-head comparison of DPDPE ([D-Pen2, D-Pen5]-enkephalin) and TFF3 (Trefoil Factor 3 (intestinal trefoil factor)) — benefits, dosing, side effects, research data, and where to buy.

Research Score

TFF3
4.1
PropertyDPDPETFF3
CategoryPain ManagementGastrointestinal
Full Name[D-Pen2, D-Pen5]-enkephalinTrefoil Factor 3 (intestinal trefoil factor)
Molecular Weight645.79 Da6370 Da
Half-Life15-60 minutes in plasmaunknown; likely short in circulation
Amino Acids559
Typical Dose0.01-1 ug per animalresearch-only; model-dependent microgram to milligram ranges
RouteIntrathecal / intracerebroventricularOral/Intrarectal
Purity≥98%≥98%
Studies Count80070
Research StatusPre-clinicalPre-clinical

DPDPE Benefits

  • delta-opioid selectivity
  • improved conformational stability
  • analgesic receptor mapping
  • useful in chronic pain models

TFF3 Benefits

  • epithelial-restitution
  • mucus-support
  • wound-healing
  • barrier-stability

DPDPE Dosing

Intrathecal 0.01-1 ug per animal|Intracerebroventricular 0.01-0.5 ug per animal|In vitro 0.1-20 nM

TFF3 Dosing

preclinical dosing varies widely by model|local or systemic administration|repeated dosing for inflammatory injury studies

DPDPE Side Effects

  • sedation
  • nausea-like opioid effects
  • tolerance with repeated dosing

TFF3 Side Effects

  • limited human dosing data
  • protein stability issues
  • potential immunogenicity with exogenous use

Research Overview

DPDPE

DPDPE is widely cited in opioid pharmacology because it helped establish the functional role of delta-opioid receptors in pain control. Its constrained structure is also used to explore how peptide cyclization changes potency, stability, and tissue selectivity.

TFF3

TFF3 is a well-established mucosal repair factor in the literature, especially in the context of intestinal injury and inflammation. Experimental studies support a role in accelerating restitution and protecting the epithelial surface in gut disease models.

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Common Stacking Partners

DPDPE stacks with:

morphinegabapentin

TFF3 stacks with:

probioticsmesalaminezinc-carnosine
cyclic-peptidedelta-opioidanalgesiaenkephalin-analogIBDmucosal-healingepithelial-restitutionmucusbarrier-protection