Leu-enkephalin vs Met-enkephalin

Head-to-head comparison of Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu) and Met-enkephalin (Tyr-Gly-Gly-Phe-Met) — benefits, dosing, side effects, research data, and where to buy.

Research Score

PropertyLeu-enkephalinMet-enkephalin
CategoryPain ManagementPain Management
Full NameTyr-Gly-Gly-Phe-LeuTyr-Gly-Gly-Phe-Met
Molecular Weight555.63 Da573.67 Da
Half-Life1-5 minutes in plasma1-5 minutes in plasma
Amino Acids55
Typical Dose0.1-5 ug per animal0.1-5 ug per animal
RouteIntrathecal / intracerebroventricularIntrathecal / intracerebroventricular
Purity≥98%≥98%
Studies Count10001200
Research StatusPre-clinicalPre-clinical

Leu-enkephalin Benefits

  • delta-opioid activity
  • endogenous analgesic model
  • receptor-selective pharmacology
  • nociception pathway research

Met-enkephalin Benefits

  • endogenous opioid signaling
  • analgesic reference ligand
  • spinal antinociception
  • neurochemical pain-pathway probe

Leu-enkephalin Dosing

Intrathecal 0.1-5 ug per animal|Intracerebroventricular 0.1-2 ug per animal|In vitro 1-100 nM

Met-enkephalin Dosing

Intrathecal 0.1-5 ug per animal|Intracerebroventricular 0.1-2 ug per animal|In vitro 1-100 nM

Leu-enkephalin Side Effects

  • sedation
  • constipation-like opioid effects
  • very short plasma stability

Met-enkephalin Side Effects

  • sedation at higher exposure
  • constipation-like opioid effects
  • rapid enzymatic degradation

Research Overview

Leu-enkephalin

Leu-enkephalin is a standard tool in opioid receptor and pain-transmission research because it helps distinguish delta- from mu-preferring signaling. Its research value is high, but its clinical utility is limited by rapid enzymatic breakdown and delivery challenges.

Met-enkephalin

Met-enkephalin has been extensively used in receptor pharmacology and nociception studies to map mu and delta opioid biology. The main limitation in translational work is very short half-life from peptidase cleavage and poor systemic stability.

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Common Stacking Partners

Leu-enkephalin stacks with:

gabapentinketamine

Met-enkephalin stacks with:

gabapentinketamine
opioid-peptideenkephalindelta-opioidanalgesiamu-opioid