Leu-enkephalin vs DPDPE

Head-to-head comparison of Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu) and DPDPE ([D-Pen2, D-Pen5]-enkephalin) — benefits, dosing, side effects, research data, and where to buy.

Research Score

PropertyLeu-enkephalinDPDPE
CategoryPain ManagementPain Management
Full NameTyr-Gly-Gly-Phe-Leu[D-Pen2, D-Pen5]-enkephalin
Molecular Weight555.63 Da645.79 Da
Half-Life1-5 minutes in plasma15-60 minutes in plasma
Amino Acids55
Typical Dose0.1-5 ug per animal0.01-1 ug per animal
RouteIntrathecal / intracerebroventricularIntrathecal / intracerebroventricular
Purity≥98%≥98%
Studies Count1000800
Research StatusPre-clinicalPre-clinical

Leu-enkephalin Benefits

  • delta-opioid activity
  • endogenous analgesic model
  • receptor-selective pharmacology
  • nociception pathway research

DPDPE Benefits

  • delta-opioid selectivity
  • improved conformational stability
  • analgesic receptor mapping
  • useful in chronic pain models

Leu-enkephalin Dosing

Intrathecal 0.1-5 ug per animal|Intracerebroventricular 0.1-2 ug per animal|In vitro 1-100 nM

DPDPE Dosing

Intrathecal 0.01-1 ug per animal|Intracerebroventricular 0.01-0.5 ug per animal|In vitro 0.1-20 nM

Leu-enkephalin Side Effects

  • sedation
  • constipation-like opioid effects
  • very short plasma stability

DPDPE Side Effects

  • sedation
  • nausea-like opioid effects
  • tolerance with repeated dosing

Research Overview

Leu-enkephalin

Leu-enkephalin is a standard tool in opioid receptor and pain-transmission research because it helps distinguish delta- from mu-preferring signaling. Its research value is high, but its clinical utility is limited by rapid enzymatic breakdown and delivery challenges.

DPDPE

DPDPE is widely cited in opioid pharmacology because it helped establish the functional role of delta-opioid receptors in pain control. Its constrained structure is also used to explore how peptide cyclization changes potency, stability, and tissue selectivity.

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Common Stacking Partners

Leu-enkephalin stacks with:

gabapentinketamine

DPDPE stacks with:

morphinegabapentin
opioid-peptideenkephalindelta-opioidanalgesiacyclic-peptideenkephalin-analog