Endomorphin-1 vs DADLE

Head-to-head comparison of Endomorphin-1 (Tyr-Pro-Trp-Phe-NH2) and DADLE ([D-Ala2, D-Leu5]-enkephalin) — benefits, dosing, side effects, research data, and where to buy.

Research Score

PropertyEndomorphin-1DADLE
CategoryPain ManagementPain Management
Full NameTyr-Pro-Trp-Phe-NH2[D-Ala2, D-Leu5]-enkephalin
Molecular Weight610.71 Da569.67 Da
Half-LifeMinutes in plasma5-20 minutes in plasma
Amino Acids45
Typical Dose0.1-10 ug per animal0.01-2 ug per animal
RouteIntrathecal / subcutaneousIntrathecal / intracerebroventricular
Purity≥98%≥98%
Studies Count600450
Research StatusPre-clinicalPre-clinical

Endomorphin-1 Benefits

  • mu-opioid selectivity
  • potent antinociception
  • endogenous peptide scaffold
  • useful for receptor-discovery studies

DADLE Benefits

  • delta-opioid agonism
  • enhanced peptide stability
  • spinal analgesia models
  • research on tolerance mechanisms

Endomorphin-1 Dosing

Intrathecal 0.1-10 ug per animal|Subcutaneous 0.1-5 ug per animal|In vitro 0.1-100 nM

DADLE Dosing

Intrathecal 0.01-2 ug per animal|Intracerebroventricular 0.01-1 ug per animal|In vitro 0.1-50 nM

Endomorphin-1 Side Effects

  • respiratory depression at high exposure
  • sedation
  • tolerance and dependence potential

DADLE Side Effects

  • sedation
  • motor suppression at higher doses
  • opioid tolerance with repeated exposure

Research Overview

Endomorphin-1

Endomorphin-1 is a key research peptide in studies of endogenous mu-opioid signaling and spinal analgesia. Like other peptide analgesics, its main translational hurdles are enzymatic instability, limited oral bioavailability, and delivery across barriers.

DADLE

DADLE has been studied for analgesic, neuroprotective, and receptor-selective opioid effects in animal and tissue models. It remains important as a mechanistic probe because it is more stable than endogenous enkephalins but still peptide-like in its delivery constraints.

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Common Stacking Partners

Endomorphin-1 stacks with:

gabapentinketamine

DADLE stacks with:

morphineketamine
mu-opioidendogenous-peptideanalgesiapain-modulationenkephalin-analogdelta-opioidpeptide-therapeutic